Thirty-nine female with SLE meeting the American College of Rheumatology 1997 criteria for SLE, aged 16-47 years, SLEDAI score >5, disease duration 2 months-96 months, average consumption of steroids (Methylprednisolone <50 mg/day), and use at least one type of immunosupresant agent were studied after giving informed consent. SLE patients who are pregnant or breast-feeding, anemia, cancer, impaired kidney function, using hormone replacement therapy, consume folic acid and vitamin B supplements, anticonvulsants agent, carbamazepine, phenytoin, xanthopterin, tamoxifen, and theophylline were excluded from the study. Subjects were obtained from Rheumatology Clinic of Dr. Saiful Anwar Hospital, Malang, Indonesia. Twelve healthy, age matched premenopausal women (medical students, resident, and healthy blood donors from the same geographical area) were studied as a control group after giving informed consent. This study used an observational analytic cross sectional approach and it was conducted in .
5 cc out-of venous blood products have been collected inside test-tube that have aple is centrifuged at the 3000 rpm for five minute. The newest demeure with no indication of hemolysis try stored in tubes within temperature out of -70°C for further analysis.
Individuals laboratory details also solution homocysteine, folic acid, supplement B6, nutritional B12, bCTx, osteocalcin, and RANKL was in fact counted by the enzyme-linked immunosorbent assay (ELISA) approach. The second parameters was basically measured of the commercial ELISA Equipment: homocysteine (NovaTeinBio Person ELISA System Overall Hcy), folic acid (during the B6 (into the B12 (Vit B12 NovaTeinBio Peoples ELISA Package), bCTx (Cusabio bCTx Peoples ELISA Equipment), osteocalcin (NovaTeinBio Peoples ELISA System Osteocalcin), and you will RANKL (NovaTeinBio People ELISA Equipment RANKL). The level of MDA try measured from the Thiobarbituric Acid Reactive Material (TBARS) method.
Mathematical study is actually performed from the SPSS to possess Screen adaptation 16. Numerical parameters had been advertised with respect to suggest and you may fundamental deviation. Within this research, parameters appearing p-value less than 0.05 was considered statistically high respectively. Pearson correlation attempt was applied to check brand new correlation.
Per diligent, study had been registered into age, SLE problem pastime list (SLEDAI) score, situation period, glucocorticoid amount, immunosuppressant agent, and latest systematic manifestations (Table step 1).
The latest imply years is 30 years having a vary from sixteen to 47 age in addition to suggest state stage try 26 weeks which have a range from dos to help you 96 months. During the study most SLE patients got only lighter problem interest as expressed of the SLEDAI six – twenty six. The SLE patients was recieved corticosteroid procedures on mean glucocorticoid amount are 26 mg/big date with a range cuatro – 48 mg/time. Nineteen SLE customers was basically obtained immunosuppresant representatives. The most common condition manifestations were kidney infection, malar rash, and you can arthritis.
The fresh suggest amounts of homocysteine, bCTx, osteocalcin, folic acidic, vitamin B6, vitamin B12, RANKL, and you can MDA into the SLE diligent and you will control group was basically demonstrated inside the Table dos.
Solution was split and accumulated inside the polythene pipe which have cork
This study unearthed that somewhat higher degrees of homocysteine was in fact discover for the SLE people (p=0.010). There can be in addition to a considerably sophisticated from MDA and you may RANKL from inside the SLE diligent (p=0.042, p=0.030). However, folic acidic, supplement B6, nutritional B12, bCTX, and you will osteocalcin accounts just weren’t statistically more anywhere between SLE clients and you may manage category.
The brand new relationship ranging from details is actually noticed just into the SLE clients. From the correlation investigation i unearthed that there had been self-confident and you may significant relationship was observed anywhere between gel level of homocysteine and bCTx (p=0.000, r=0.943). Another positive and significant correlations was basically as well as discover between gel top away from homocysteine and you will MDA (p=0.002, r=0.731). Which quantity of MDA possess tall and you will positive relationship that have RANKL (p=0.one hundred thousand, r=0.758).
Bad and you will high correlations was basically noticed ranging from homocysteine and you can osteocalcin (p=0.100, r=-0.771), homocysteine and folic acidic (p=0.100, r=-0.734), and homocysteine and you may nutritional B6 (p=0.046, r=-0.332). 080, r=-0.284) (Dining table 3).